HORIZON: An Alternative Method for Solubility Measurement
Aqueous solubility of small molecule compounds is an essential parameter in drug discovery and development. Low solubility can impact biological assays, leading to underestimated potency and toxicity, inaccurate SAR, and difficult to interpret ADME results. Despite its critical importance at early stages, common detection methods for solubility measurement are inefficient, lack sensitivity, or are sensitive to sample processing.
The HORIZON® system with Backgrounded Membrane Imaging (BMI) is a subvisible particle analysis system that can measure compound solubility in high throughput via a simple and flexible workflow. In addition, highly resolved particle images provide valuable information on morphology of precipitated solids for more comprehensive solubility characterization.
Low Volume, High Throughput Subvisible Aggregate Analysis
BMI is a novel automated microscopy technology that images and analyzes insoluble aggregates captured on a membrane in low-volume, high throughput format. It offers the ease of use and throughput advantages homogeneous solution assays but with higher sensitivity and more comprehensive particle data. Since aggregates are measured on a membrane surface and not in solution, presence of solvent, matrix effects, or compound stickiness are not a concern with BMI. Every particle ≥2 μm is counted and analyzed, regardless of particle number or size distribution, so that changes in solubility can be detected even when only a few aggregates are present in the sample.